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Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells
Chapters
- 00:04Introduction
- 00:50Incisor Dissection and Isolation of the Proximal End Tissue
- 03:45Tissue Fixation
- 04:19Results: Analysis of the Isolated Cervical Loops
- 05:51Conclusion
The Trowell-type organ culture method has been used to unravel complex signaling networks that govern tooth development and, more recently, for studying regulation involved in stem cells of the continuously growing mouse incisor. Fluorescent-reporter animal models and live-imaging methods facilitate in-depth analyses of dental stem cells and their specific niche microenvironment.
