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使用膜结合IL-21修饰B细胞系的自然杀伤（NK）和CAR-NK细胞扩增方法
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February 08, 2022
DOI:
1Department of Pathology, Immunology and Laboratory Medicine,Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School,Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School,Rutgers-The State University of New Jersey
Summary
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在这里，我们提出了一种扩增外周血自然杀伤（PBNK），来自肝组织的NK细胞以及源自外周血单核细胞（PBMC）或脐带血（CB）的嵌合抗原受体（CAR）-NK细胞的方法。该协议展示了使用221-mIL-21饲养层细胞的NK和CAR-NK细胞的扩增，以及扩增的NK细胞的优化纯度。
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Ma, M., Badeti, S., Kim, J. K., Liu, D. Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line. J. Vis. Exp. (180), e62336, doi:10.3791/62336 (2022).
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