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Fixed Target Serial Data Collection at Diamond Light Source
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JoVE 신문 생화학
Fixed Target Serial Data Collection at Diamond Light Source

Fixed Target Serial Data Collection at Diamond Light Source

DOI:
10.3791/62200-v

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06:19 min

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February 26, 2021

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, , , , , , , , ,
1Diamond Light Source, Harwell Science and Innovation Campus, 2School of Life Sciences,University of Essex, 3X-ray Science Division,Argonne National Laboratory, 4European Molecular Biology Laboratory, Hamburg Outstation c/o DESY, 5Biological Sciences, Institute for Life Sciences,University of Southampton

Chapters

  • 00:04Introduction
  • 00:59Preparing and Loading a Chip
  • 02:53Aligning the Chip
  • 04:18Results: Data Indexing and Integration and Visualization of Unit Cell Parameters
  • 05:30Conclusion

Summary

자동 번역

자동 번역

We present a comprehensive guide to fixed target sample preparation, data collection, and data processing for serial synchrotron crystallography at Diamond beamline I24.

Tags

Serial Data CollectionFixed TargetSerial Synchrotron CrystallographySSXLow DoseRoom TemperatureProtein DynamicsSilicon ChipPolyester FoilChip HolderChip LoadingMicrocrystal SlurryVacuumAlignmentKinematic MountXYZ StageOn-axis Viewing

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