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Cristallizzazione e determinazione strutturale di un enzima:complesso di substrati mediante cristallografia seriale in un chip microfluidico versatile
JoVE 신문
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JoVE 신문 생화학
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
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Cristallizzazione e determinazione strutturale di un enzima:complesso di substrati mediante cristallografia seriale in un chip microfluidico versatile

6,968 Views

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10:45 min

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March 20, 2021

DOI:

10.3791/61972-v

DOI:
10.3791/61972-v

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10:45 min
March 20, 2021

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9 Views
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1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS,Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry,Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH

Tags

CrystallizationStructural DeterminationEnzyme-substrate ComplexSerial CrystallographyMicrofluidic ChipCounter-diffusionIn Situ X-ray DiffractionBiomoleculeCrystal GrowthRoom Temperature Analysis

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de Wijn, R., Rollet, K., Olieric, V., Hennig, O., Thome, N., Noûs, C., Paulus, C., Lorber, B., Betat, H., Mörl, M., Sauter, C. Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip. J. Vis. Exp. (169), e61972, doi:10.3791/61972 (2021).

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