Journal
/
생화학
/
Kristallisation und strukturelle Bestimmung eines Enzyms: Substratkomplex durch serielle Kristallisation in einem vielseitigen mikrofluidischen Chip
JoVE 신문
생화학
Author Produced
This content is Free Access.
JoVE 신문 생화학
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
Please note that all translations are automatically generated. Click here for the English version.

Kristallisation und strukturelle Bestimmung eines Enzyms: Substratkomplex durch serielle Kristallisation in einem vielseitigen mikrofluidischen Chip

6,968 Views

•

10:45 min

•

March 20, 2021

DOI:

10.3791/61972-v

DOI:
10.3791/61972-v

•

10:45 min
March 20, 2021

•

9 Views
, , , , , , , , , ,
1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS,Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry,Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH

Tags

CrystallizationStructural DeterminationEnzyme-substrate ComplexSerial CrystallographyMicrofluidic ChipCounter-diffusionIn Situ X-ray DiffractionBiomoleculeCrystal GrowthRoom Temperature Analysis

Read Article

Cite this Article

de Wijn, R., Rollet, K., Olieric, V., Hennig, O., Thome, N., Noûs, C., Paulus, C., Lorber, B., Betat, H., Mörl, M., Sauter, C. Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip. J. Vis. Exp. (169), e61972, doi:10.3791/61972 (2021).

Download .ris file

Copy

Share Video

.

Copy