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05:52 min
November 25, 2020
DOI:
10.3791/61868-v
该协议描述了小鼠 神经视网膜的细胞图外植,与视网膜色素上皮(RPE)一起在R16定义的介质中培养,不含血清和抗生素。与 体内 实验相比,这种方法性能相对简单、成本更低、耗时,可适应多种实验应用。
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Belhadj, S., Tolone, A., Christensen, G., Das, S., Chen, Y., Paquet-Durand, F. Long-Term, Serum-Free Cultivation of Organotypic Mouse Retina Explants with Intact Retinal Pigment Epithelium. J. Vis. Exp. (165), e61868, doi:10.3791/61868 (2020).
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