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长期,无血清培养有机小鼠视网膜外植与完整的视网膜色素上皮
JoVE 신문
신경과학
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JoVE 신문 신경과학
Long-Term, Serum-Free Cultivation of Organotypic Mouse Retina Explants with Intact Retinal Pigment Epithelium

长期,无血清培养有机小鼠视网膜外植与完整的视网膜色素上皮

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05:52 min

November 25, 2020

DOI:

05:52 min
November 25, 2020

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Summary

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该协议描述了小鼠 神经视网膜的细胞图外植,与视网膜色素上皮(RPE)一起在R16定义的介质中培养,不含血清和抗生素。与 体内 实验相比,这种方法性能相对简单、成本更低、耗时,可适应多种实验应用。

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