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Aislamiento de células CD8 + T residentes en el riñón delratón para el análisis de citometría de flujo
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JoVE 신문 면역학 및 감염병학
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
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Aislamiento de células CD8 + T residentes en el riñón delratón para el análisis de citometría de flujo

6,152 Views

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06:07 min

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June 27, 2020

DOI:

10.3791/61559-v

DOI:
10.3791/61559-v

•

06:07 min
June 27, 2020

•

11 Views
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1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine,University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital,Central South University

Summary

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Después de la infección viral, el riñón alberga un número relativamente grande de células CD8+ T y ofrece la oportunidad de estudiar las células TRM no mucosas. Aquí, describimos un protocolo para aislar linfocitos renales de ratón para el análisis de citometría de flujo.

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Cite this Article

Liao, W., Ma, C., Zhang, N. Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis. J. Vis. Exp. (160), e61559, doi:10.3791/61559 (2020).

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