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Isolement des cellules CD8+ T résidentes du rein de souris pour l’analyse de cytométrie de flux
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JoVE 신문 면역학 및 감염병학
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
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Isolement des cellules CD8+ T résidentes du rein de souris pour l’analyse de cytométrie de flux

6,152 Views

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06:07 min

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June 27, 2020

DOI:

10.3791/61559-v

DOI:
10.3791/61559-v

•

06:07 min
June 27, 2020

•

11 Views
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1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine,University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital,Central South University

Summary

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Suite à une infection virale, le rein abrite un nombre relativement important de cellules CD8+ T et offre la possibilité d’étudier les lymphocytes TRM non muqueux. Ici, nous décrivons un protocole pour isoler les lymphocytes rénaux de souris pour l’analyse de cytométrie de flux.

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Cite this Article

Liao, W., Ma, C., Zhang, N. Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis. J. Vis. Exp. (160), e61559, doi:10.3791/61559 (2020).

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