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Extractie en kwantificering van oplosbare, radiogelabelde Inositol Polyfosfaten van verschillende plantensoorten met SAX-HPLC
JoVE 신문
생화학
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JoVE 신문 생화학
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
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Extractie en kwantificering van oplosbare, radiogelabelde Inositol Polyfosfaten van verschillende plantensoorten met SAX-HPLC

6,541 Views

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09:01 min

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June 26, 2020

DOI:

10.3791/61495-v

DOI:
10.3791/61495-v

•

09:01 min
June 26, 2020

•

11 Views
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1Department of Plant Nutrition, Institute of Crop Science and Resource Conservation,University of Bonn

Summary

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Hier beschrijven we sterke anion uitwisseling high-performance vloeibare chromatografie van [3H]-myo-inositol-gelabelde zaailingen die een zeer gevoelige methode is om inositol polyfosfaten in planten te detecteren en te kwantificeren.

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Cite this Article

Gaugler, P., Gaugler, V., Kamleitner, M., Schaaf, G. Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC. J. Vis. Exp. (160), e61495, doi:10.3791/61495 (2020).

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