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Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
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Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

6,560 Views

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09:01 min

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June 26, 2020

DOI:

10.3791/61495-v

DOI:
10.3791/61495-v

•

09:01 min
June 26, 2020

•

11 Views
, , ,
1Department of Plant Nutrition, Institute of Crop Science and Resource Conservation,University of Bonn

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Here we describe strong anion exchange high-performance liquid chromatography of [3H]-myo-inositol-labeled seedlings which is a highly sensitive method to detect and quantify inositol polyphosphates in plants.

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Inositol PolyphosphatesRadiolabelingSAX-HPLCPlant MetabolismPlant DevelopmentExtractionQuantificationLotus SeedsMyo-inositolSample PreparationHPLC Setup

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Gaugler, P., Gaugler, V., Kamleitner, M., Schaaf, G. Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC. J. Vis. Exp. (160), e61495, doi:10.3791/61495 (2020).

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