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胚胎微注射技术，用于在库莱克斯五角星高效位点特异性突变体
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Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus
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胚胎微注射技术，用于在库莱克斯五角星高效位点特异性突变体

4,961 Views

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05:59 min

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May 24, 2020

DOI:

10.3791/61375-v

DOI:
10.3791/61375-v

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05:59 min
May 24, 2020

•

7 Views
, , , ,
1Section of Cell and Developmental Biology,University of California, San Diego, 2Department of Entomology and Plant Patholog,Auburn University, 3Tata Institute for Genetics and Society,University of California, San Diego

Summary

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该协议描述了 Culex五角星胚胎的 微注射程序，这些胚胎通过CRISPR/Cas9基因编辑工具进行优化。该技术可以有效地生成特定地点、遗传的生殖系突变，可用于在这种研究不足的疾病载体中构建遗传技术。

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Cite this Article

Bui, M., Li, M., Raban, R. R., Liu, N., Akbari, O. S. Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus. J. Vis. Exp. (159), e61375, doi:10.3791/61375 (2020).

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