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Digestión de ojos enteros del ratón para el análisis citométrico de flujo multiparamétrico de fagocitos mononucleares
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
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Digestión de ojos enteros del ratón para el análisis citométrico de flujo multiparamétrico de fagocitos mononucleares

4,704 Views

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09:58 min

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June 17, 2020

DOI:

10.3791/61348-v

DOI:
10.3791/61348-v

•

09:58 min
June 17, 2020

•

2 Views
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1Department of Ophthalmology, Feinberg School of Medicine,Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine,Northwestern University

Summary

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Este protocolo proporciona un método para digerir los ojos enteros en una sola suspensión celular con el propósito de análisis citométrico de flujo multiparamétrico con el fin de identificar poblaciones fagocíticas mononucleares oculares específicas, incluyendo monocitos, microglia, macrófagos y células dendríticas.

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Cite this Article

Droho, S., Cuda, C. M., Lavine, J. A. Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes. J. Vis. Exp. (160), e61348, doi:10.3791/61348 (2020).

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