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1Translational Vascular Medicine Branch, National Heart Lung and Blood Institute,National Institutes of Health, 2Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke,National Institutes of Health, 3Division of Pediatric Surgery, Department of Surgery,University of North Carolina at Chapel Hill
Chapters
- 00:04Introduction
- 00:52Pulmonary Artery (PA) Catheterization and Blood Perfusion
- 03:18Tracheostomy and Lung Inflation
- 04:59Casting the Vasculature
- 06:44Results: Representative Successful Vascular Casting and Common Errors during Polymer Compound Infusion
- 08:24Conclusion
El objetivo de esta técnica es la visualización ex vivo de redes arteriales pulmonares de ratones postnatales y adultos tempranos a través de la inflación pulmonar y la inyección de un compuesto radioopaco a base de polímeros a través de la arteria pulmonar. También se discuten las posibles aplicaciones para los tejidos fundidos.
