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1Translational Vascular Medicine Branch, National Heart Lung and Blood Institute,National Institutes of Health, 2Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke,National Institutes of Health, 3Division of Pediatric Surgery, Department of Surgery,University of North Carolina at Chapel Hill
Chapters
- 00:04Introduction
- 00:52Pulmonary Artery (PA) Catheterization and Blood Perfusion
- 03:18Tracheostomy and Lung Inflation
- 04:59Casting the Vasculature
- 06:44Results: Representative Successful Vascular Casting and Common Errors during Polymer Compound Infusion
- 08:24Conclusion
Das Ziel dieser Technik ist die ex vivo Visualisierung von pulmonalen arteriellen Netzwerken von frühpostnatalen und erwachsenen Mäusen durch Lungeninflation und Injektion einer radio-opaque polymerbasierten Verbindung über die Lungenarterie. Mögliche Anwendungen für gegossenes Gewebe werden ebenfalls diskutiert.
