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Chemistry

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JoVE Journal Chemistry
Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))
 

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))

Article DOI: 10.3791/61055-v • 04:48 min • May 4th, 2020
May 4th, 2020 •
1, 1,4, 2,3
1Research Unit Analytical BioGeoChemistry, Helmholz Zentrum München-German Research Center for Environmental Health, 2Department of Hematology and Medical Oncology, University Medical Center Göttingen (UMG), 3Institute of Pathology, University Medical Center Göttingen (UMG), 4RECIPE Chemicals + Instruments GmbH

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요약
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This iron redox speciation method is based on capillary electrophoresis-inductively coupled plasma mass spectrometry with sample stacking combined with short analysis in one run. The method quickly analyzes and provides low limits of quantification for iron redox species across a diverse range of tissues and biofluid samples.

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Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS Iron Redox Species Fe(II) Fe(III) Excessive Iron Oxidative Stress Ferroptosis Quantitative Measurements Fast Analysis Low Quantification Limits Nebulizer Homemade Interface Luer Connectors Platinum Wire Silicone Tube CE Capillary
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