Here, standardized protocols are presented to assess induction of the heat shock response (HSR) in Caenorhabditis elegans using RT-qPCR at the molecular level, fluorescent reporters at the cellular level, and thermorecovery at the organismal level.
Golden, N. L., Plagens, R. N., Kim Guisbert, K. S., Guisbert, E. Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans. J. Vis. Exp. (161), e61030, doi:10.3791/61030 (2020).