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使用χCRAC以高时间分辨率监测体内蛋白质-RNA相互作用动力学
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Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
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使用χCRAC以高时间分辨率监测体内蛋白质-RNA相互作用动力学

4,504 Views

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09:15 min

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May 09, 2020

DOI:

10.3791/61027-v

DOI:
10.3791/61027-v

•

09:15 min
May 09, 2020

•

33 Views
, , , , , , , , ,
1Centre for Engineering Biology,University of Edinburgh, 2Institute of Cell Biology,University of Edinburgh, 3Department of Molecular Biology and Genetics,Aarhus University, 4UVO3 Ltd.

Summary

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cDNA的动力学交联和分析是一种允许以高时间分辨率研究活细胞中蛋白质-RNA相互作用动力学的方法。这里详细描述了该方案，包括酵母细胞的生长、UV交联、收获、蛋白质纯化和下一代测序文库的制备步骤。

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McKellar, S. W., Ivanova, I., van Nues, R. W., Cordiner, R. A., Chauhan, M., Christopoulou, N., Worboys, W., Langford, A., Jensen, T. H., Granneman, S. Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC. J. Vis. Exp. (159), e61027, doi:10.3791/61027 (2020).

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