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A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons
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A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

DOI:
10.3791/60955-v

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07:41 min

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August 06, 2020

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, , , ,
1Department of Molecular Genetics, Faculty of Biological Sciences,Tarbiat Modares University, 2Center for Therapeutic Innovation and Department of Psychiatry & Behavioral Sciences,University of Miami Miller School of Medicine, 3Department of Biochemistry and Molecular Biology,University of Miami Miller School of Medicine, 4Persian BayanGene Research and Training Center

Chapters

  • 00:04Introduction
  • 00:51Passaging and Freezing the hNPCs
  • 02:50Image Acquisition and Analysis
  • 04:25Neurotoxicity Assessment
  • 06:10Results: Neurite Outgrowth Assay and Neurotoxicity Assessment with Small Molecule Compounds
  • 07:13Conclusion

Summary

자동 번역

자동 번역

The presented protocol describes a method for a neurite outgrowth assay and neurotoxicity assessment of small molecule compounds.

Tags

Neurite Outgrowth AssayNeurotoxicity AssessmentHuman Neural Progenitor CellsNeuronsInduced Pluripotent Stem CellsEmbryonic Stem CellsNeurosphereCell DissociationCell CultureCryopreservationImage AnalysisFijiROI ManagerFreehand Line

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