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Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)
1Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences, 2Artemisinin Research Center,China Academy of Chinese Medical Sciences, 3College of Life Science,Huaibei Normal University, 4Economic Crop Research Institute Sichuan Academy of Agriculture Sciences, 5Research Center of Buckwheat Industry Technology,Guizhou Normal University
Chapters
- 00:04Introduction
- 01:01Tartary Buckwheat (TB) Seed Preparation
- 01:49TB Seedling and Explant Preparation
- 02:46A. rhizogenes Transformation Preparation
- 03:56TB Explant Screening, Infection, and Co-Culture
- 04:47Induction and Selective Culture and TB Hairy Root Subculture
- 05:32Transformed Hairy Root Identification and Conservation
- 06:14Results: Representative Hairy Root Transformation Identification and Characterization
- 07:23Conclusion
We describe a method of inducing hairy roots by Agrobacterium rhizogenes-mediated transformation in Tartary buckwheat (Fagopyrum tataricum). This can be used to investigate gene functions and production of secondary metabolites in Tartary buckwheat, be adopted for any genetic transformation, or used for other medicinal plants after improvement.
