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神経紋の分離、培養、脂肪形成誘導、周食脂肪組織由来の脂肪由来幹細胞
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March 02, 2020
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1Department of Cardiology, Shanghai Chest Hospital,Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences,Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension,Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine,Huadong Hospital Affiliated to Fudan University
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Wnt-1 Cre+/-の周産脂肪組織から誘導された脂肪由来幹細胞 (NCAC) の神経堤の分離、培養、脂肪誘導のプロトコルを提示します。ローザ26RFP/+マウス。NCAdscは、インビトロで脂肪形成または脂肪形成をモデル化するためのACの簡単にアクセス可能なソースであることができます。
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Cite this Article
Qi, Y., Miao, X., Xu, L., Fu, M., Peng, S., Shi, K., Li, J., Ye, M., Li, R. Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue. J. Vis. Exp. (157), e60691, doi:10.3791/60691 (2020).
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