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使用嘧啶类似物5-碘-2′-脱氧尿苷（IdU）和细胞周期标志物在质谱细胞术平台中建立细胞周期期
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Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform
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使用嘧啶类似物5-碘-2′-脱氧尿苷（IdU）和细胞周期标志物在质谱细胞术平台中建立细胞周期期

DOI:
10.3791/60556-v

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08:37 min

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October 22, 2021

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,
1Ohio State Comprehensive Cancer Center, Division of Hematology,The Ohio State University

Chapters

  • 00:04Introduction
  • 00:515‐Iodo‐2’‐Deoxyuridine (IdU) Incorporation and Sample Preservation
  • 02:28Data Analysis
  • 06:27Results: Representative Cell Cycle Phase Establishment
  • 07:55Conclusion

Summary

자동 번역

자동 번역

该协议调整细胞周期测量以用于质质细胞术平台。凭借质谱细胞术的多参数功能，直接测量碘掺入可以鉴定S期细胞，而细胞内循环标记物可以在一系列实验条件下表征每个细胞周期状态。

Tags

Cell CycleMass Cytometry5-Iodo-2′-Deoxyuridine (IdU)Cell Cycle MarkersAcute Myeloid LeukemiaStem And Progenitor CellsCell GrowthCell ProliferationLive/dead StainParaformaldehydeCell Staining MediumDMSOIridium

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