Journal
/
유전학
/
Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform

Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform

DOI:
10.3791/60556-v

•

08:37 min

•

October 22, 2021

•

,
1Ohio State Comprehensive Cancer Center, Division of Hematology,The Ohio State University

Chapters

  • 00:04Introduction
  • 00:515‐Iodo‐2’‐Deoxyuridine (IdU) Incorporation and Sample Preservation
  • 02:28Data Analysis
  • 06:27Results: Representative Cell Cycle Phase Establishment
  • 07:55Conclusion

Summary

자동 번역

자동 번역

This protocol adapts cell cycle measurements for use in a mass cytometry platform. With the multi-parameter capabilities of mass cytometry, direct measurement of iodine incorporation allows identification of cells in S-phase while intracellular cycling markers enable characterization of each cell cycle state in a range of experimental conditions.

Tags

Cell CycleMass Cytometry5-Iodo-2′-Deoxyuridine (IdU)Cell Cycle MarkersAcute Myeloid LeukemiaStem And Progenitor CellsCell GrowthCell ProliferationLive/dead StainParaformaldehydeCell Staining MediumDMSOIridium

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Detection of Copy Number Alterations Using Single Cell Sequencing

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

Read Article