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胸腔内注射治疗成年斑马鱼心脏的研究
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JoVE 신문 생물학
Intrathoracic Injection for the Study of Adult Zebrafish Heart
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胸腔内注射治疗成年斑马鱼心脏的研究

DOI:
10.3791/59724-v

•

07:22 min

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May 14, 2019

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,
1Department of Biology,University of Fribourg

Chapters

  • 00:04Title
  • 00:35Tools and Solutions for Injections
  • 01:30Preparation of the Injection Station
  • 02:28Preparation of the Fish for Intrathoracic Injection
  • 03:04Microinjection into the Pericardium
  • 04:07Results: Validation and Analysis of the Intrathoracic Injection Method
  • 06:35Conclusion

Summary

자동 번역

자동 번역

这种方法依赖于在成年斑马鱼的胸部注射0.5-3Μl 溶液。该程序有效地将蛋白质和化合物输送到斑马鱼心脏附近, 而不会损害器官。该方法适用于检测外源因素对心脏各组织的影响。

Tags

Intrathoracic InjectionAdult Zebrafish HeartCardiac ResearchDrug DeliveryMicroinjectionAnesthesiaFish Preparation

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