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Kvantitativ fluorescens i situ-hybridisering (fisk) och Immunofluorescensbildning (IF) av specifika genprodukter i KSHV-infekterade celler
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JoVE 신문 면역학 및 감염병학
Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells
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Kvantitativ fluorescens i situ-hybridisering (fisk) och Immunofluorescensbildning (IF) av specifika genprodukter i KSHV-infekterade celler

11,855 Views

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00:06 min

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August 27, 2019

DOI:

10.3791/59697-v

DOI:
10.3791/59697-v

•

00:06 min
August 27, 2019

•

9 Views
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1Norfolk Academy, 2Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute,Yale School of Medicine

Tags

Quantitative FISHImmunofluorescenceKSHVHost-virus InteractionsViral Gene ProductsRNAProteinBiochemical AssaysCell CultureFixationPermeabilizationBlockingPrimary AntibodySecondary AntibodyFluorescent LabelingHybridizationOligonucleotidesDenaturation

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Vallery, T. K., Steitz, J. A. Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells. J. Vis. Exp. (150), e59697, doi:10.3791/59697 (2019).

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