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将化学遗传工程皮质内神经内生前生移植到早期产后小鼠大脑
JoVE 신문
신경과학
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JoVE 신문 신경과학
Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains
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将化学遗传工程皮质内神经内生前生移植到早期产后小鼠大脑

DOI:
10.3791/59568-v

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00:06 min

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August 26, 2019

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, , ,
1Nervous System Development and Homeostasis Laboratory,The Francis Crick Institute, 2Neuroscience Centre,Biomedical Sciences Research Centre “Al. Fleming”, 3Centre for Developmental Neurobiology,King’s College London

Chapters

  • 00:04Title
  • 00:41Mouse Embryo Cortical Slice Preparation
  • 02:27Focal DNA Injection
  • 03:53Acute Brain Slice Electroporation
  • 04:54Results: Representative Cortical Interneuron Progenitor Transplantation Analyses
  • 05:48Conclusion

Summary

자동 번역

자동 번역

在这里，我们提出一个协议，旨在使用化学遗传学工具操纵移植到早期产后小鼠皮层的皮质内祖细胞的活动。

Tags

TransplantationChemogenetically EngineeredCortical Interneuron ProgenitorsPostnatal Mouse BrainCortical Interneuron PrecursorsCellular PropertiesCortical Interneuron ProgenitorsAgaroseVibrating Blade MicrotomeGanglionic EminencesFilter MembranesMinimal Essential MediumFocal DNA InjectionAgarose ColumnsElectroporation Electrode

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