Journal
/
화학
/
Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs
JoVE 신문
화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 화학
Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs

12,748 Views

•

10:44 min

•

May 15, 2019

DOI:

10.3791/59472-v

DOI:
10.3791/59472-v

•

10:44 min
May 15, 2019

•

17 Views
, , , , ,
1Department of Internal Medicine III,University Hospital Ulm, 2Pharmaceutical Institute, Pharmaceutical Chemistry I,University of Bonn

Summary

Automatically generated

This work describes the synthesis and characterization of a pomalidomide-based, bifunctional homo-PROTAC as a novel approach to induce ubiquitination and degradation of the E3 ubiquitin ligase cereblon (CRBN), the target of thalidomide analogs.

Explore More Videos

CRBN InhibitionPROTACPomalidomideCereblonE3 Ubiquitin LigaseProtein DegradationChemical SynthesisGlutarimide3-fluorophthalic AnhydrideDiamine Linker

Read Article

Cite this Article

Lindner, S., Steinebach, C., Kehm, H., Mangold, M., Gütschow, M., Krönke, J. Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs. J. Vis. Exp. (147), e59472, doi:10.3791/59472 (2019).

Download .ris file

Copy

Share Video

.

Copy