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Genetics

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JoVE Journal Genetics
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
 

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Article DOI: 10.3791/59455-v • 10:33 min • June 17th, 2019
June 17th, 2019 •
1, 1, 1,2
1Fish Health Research Group, Norwegian Veterinary Institute, 2University of Bergen

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The Multi-Locus Variable-number tandem-repeat Analysis (MLVA) assay presented here enables inexpensive, robust and portable high-resolution genotyping of the fish-pathogenic bacterium Yersinia ruckeri. Starting from pure cultures, the assay employs multiplex PCR and capillary electrophoresis to produce ten-loci MLVA profiles for downstream applications.

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Multi-locus Variable-number Tandem-repeat Analysis Fish-pathogenic Bacterium Yersinia Ruckeri Multiplex PCR Capillary Electrophoresis MLB Genotyping Protocol Epidemiology Global Population Structure Strain Resolution Diagnostic Specificity Infection Tracing Saima Nasrin Mohammad Sterile Inoculation Loops Yersinia Ruckeri Pure Cultures Petri Dishes Incubate Representative Colony Centrifuge Ultra Purified Water Heating Block
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