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1Department of Molecular Genetics and Microbiology,Duke University Medical Center, 2Department of Biotechnology and Life Science,Tokyo University of Agriculture and Technology, 3Department of Neurobiology,Duke University Medical Center, 4Department of Mechanical Systems, Engineering,Tokyo University of Agriculture and Technology, 5Institute of Global Innovation Research,Tokyo University of Agriculture and Technology, 6Duke Institute for Brain Sciences,Duke University
Chapters
- 00:04Title
- 00:42Hana3A Cells Culture
- 02:26Preparation of the Cells for Transfection
- 03:23Plasmid Transfection
- 05:01Substrate Incubation
- 06:07Odorant Stimulation and Data Analysis
- 08:07Results: Real-time Activation and Dose Dependent Responses
- 09:21Conclusion
Physiologiquement, les odorants récepteurs sont activés par des molécules odorantes inhalés en phase vapeur. Cependant, la plupart des systèmes in vitro utilisent une stimulation de la phase liquide odorant. Nous présentons ici une méthode qui permet un suivi en temps réel in vitro de l’activation des récepteurs odorants lors de la stimulation de la substance odorante en phase gazeuse.
