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用于阿尔韦拉尔巨噬细胞和CD4和T细胞免疫造血和HIV储液评估的支气管性洗浴液和匹配血液的处理
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June 23, 2019
DOI:
1Research Institute McGill University Health Centre, 2Department of Biological Sciences,Université de Québec à Montréal, 3Department of Microbiology & Immunology,McGill University, 4Department of Medicine, Division of Infectious Diseases,McGill University, 5Centre de Recherche du Centre Hospitalier de l’Université de Montréal, 6Département de microbiologie, infectiologie et immunologie,Université de Montréal
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我们描述了一种处理支气管性肠液的方法,以及通过抗逆转录病毒疗法对慢性艾滋病毒感染者进行匹配的外周血,以评估肺艾滋病毒储存。这些方法导致获得高度纯的CD4 T细胞和海泡巨噬细胞,随后可用于免疫分型和HIV DNA/RNA定量通过超敏感聚合酶链反应。
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Cite this Article
Salahuddin, S., Thomson, E., Méziane, O., Farnos, O., Pagliuzza, A., Chomont, N., Olivenstein, R., Costiniuk, C., Jenabian, M. Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment. J. Vis. Exp. (148), e59427, doi:10.3791/59427 (2019).
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