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Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
Chapters
- 00:04Title
- 00:38Collection of Ovulated Oocytes from Female Mice
- 02:06SM-FISH Staining of Oocytes
- 04:21Image Processing
- 06:21Results: mRNA Quantification Results using the Spot Finding and Tracking Program
- 07:45Conclusion
To reproducibly count the numbers of mRNAs in individual oocytes, single molecule RNA fluorescence in situ hybridization (RNA-FISH) was optimized for non-adherent cells. Oocytes were collected, hybridized with the transcript specific probes, and quantified using an image quantification software.
