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1Department of Genetics and Metabolism,Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, 2Birth Defects Prevention and Control Institute of Guangxi Zhuang Autonomous Region, 3Shenzhen Research Institute,The Chinese University of Hong Kong, 4Department of Obstetrics and Gynaecology,The Chinese University of Hong Kong, 5State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology,Shanghai Jiao Tong University, 6Basecare Medical Device Co., Ltd
Chapters
- 00:04Title
- 01:26Library Pooling and Emulsion PCR Using an Emulsion System
- 03:21Enrichment System (ES) Setup
- 04:29Template Preparation
- 05:17Sample Loading and Sequencing
- 07:56Results: Next-generation Semiconductor Sequencing for PGT-A
- 08:25Conclusion
Tags
Semiconductor SequencingPreimplantation Genetic TestingAneuploidyRapidLow-costTemplate AmplificationSequencing LibraryPGTA DetectionHigh ThroughputCost EfficientTime SavingTurnaround TimeGenome SequencingCopy Number VariationSingle Nucleotide PolymorphismSequencing ChemistryNon-invasive Prenatal ScreeningLibrary PreparationRecovery TubesRecovery RouterAmplification PlateMaster Mix PCR BufferSphere ParticlesLigation MixReaction FilterProton Program
