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Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
JoVE 신문
환경과학
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JoVE 신문 환경과학
Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
DOI:

10:09 min

February 03, 2020

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Chapters

  • 00:05Introduction
  • 01:23SEM Sample Preparation and Imaging
  • 04:40TEM Sample Preparation and Imaging
  • 08:26Results: SEM and TEM
  • 09:42Conclusion

Summary

자동 번역

In order to observe ultrastructure of insect sensilla, scanning and transmission electron microscopy (SEM and TEM, respectively) sample preparation protocol were presented in the study. Tween 20 was added into the fixative to avoid sample deformation in SEM. Fluorescence microscopy was helpful for improving slicing accuracy in TEM.

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