Journal
/
면역학 및 감염병학
/
Isolering af enkelt intracellulære bakterielle samfund genereret fra en Murine Model af urinvejsinfektion for Downstream encellede analyse
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

Isolering af enkelt intracellulære bakterielle samfund genereret fra en Murine Model af urinvejsinfektion for Downstream encellede analyse

DOI:
10.3791/58829-v

•

07:34 min

•

April 16, 2019

•

, , , , ,
1Infectious Diseases Group,Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering,National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine,National University of Singapore

Chapters

  • 00:04Title
  • 00:41Glass Capillary Preparation
  • 02:06Bladder Epithelial Cell Harvest
  • 03:53Intracellular Bacterial Community (IBC) Isolation
  • 05:18Results: Harvested IBC Are Pure and Can Be Used for Downstream Analysis
  • 06:30Conclusion

Summary

자동 번역

자동 번역

Denne protokol beskriver en simpel metode til at isolere enkelt, inficeret blæren epitel celler fra en murine model af urinvejsinfektion.

Tags

Keywords: Intracellular Bacterial CommunitiesIBCsUrinary Tract InfectionUTIMouse BladderMouth MicropipettingGlass Capillary TubeBladder Epithelial CellsSingle-cell Analysis

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/58829?article_type=v

Transurethral Induction of Mouse Urinary Tract Infection
check_url/kr/58829?article_type=v

Introducing Shear Stress in the Study of Bacterial Adhesion
check_url/kr/58829?article_type=v

RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
check_url/kr/58829?article_type=v

Infektion af zebrafisk embryoner med intracellulære bakterielle patogener
check_url/kr/58829?article_type=v

Encellede Analyse af Bacillus subtilis Biofilm Brug fluorescensmikroskopi og Flowcytometri
check_url/kr/58829?article_type=v

Isolering og genom analyse af enkelte Virioner benytter "enkelt virus Genomics '
check_url/kr/58829?article_type=v

Isolering af lymfocytter fra Mouse Genital Tract mucosa
check_url/kr/58829?article_type=v

Single Cell Målinger af vacuolær Brud forårsaget af Intracellulære patogener
check_url/kr/58829?article_type=v

Udarbejdelse af Single-celle suspensioner for cytofluorimetrisk Analyse fra forskellige musehud Regioner
check_url/kr/58829?article_type=v

Urinvejsinfektion i en lille dyremodel: transuretral kateterisation af mandlige og kvindelige mus

Read Article