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Rekombinant füzyon protein flüoresan proteaz tahlil Platform ve onların jel Renaturation kullanımı
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Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
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Rekombinant füzyon protein flüoresan proteaz tahlil Platform ve onların jel Renaturation kullanımı

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19:23 min

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January 16, 2019

DOI:

10.3791/58824-v

DOI:
10.3791/58824-v

•

19:23 min
January 16, 2019

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2 Views
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1Department of Biochemistry and Molecular Biology, Faculty of Medicine,University of Debrecen, 2Biotechnology Research Department,Gedeon Richter Plc

Summary

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Burada, N-terminal hexahistidine/maltoz-bağlayıcı protein ve nikel-nitrilotriacetic yüzeye bağlı floresan protein erimiş rekombinant yüzeylerde kullanan bir son zamanlarda geliştirilen proteaz tahlil platform ayrıntılı yordam mevcut asit manyetik özel boncuk. Bir sonraki jel analiz Sodyum Lauryl Sülfat-polyacrylamide jel elektroforez tarafından ayrılmış tahlil örnekleri de sunulmaktadır.

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Cite this Article

Bozóki, B., Mótyán, J. A., Miczi, M., Gazda, L. D., Tőzsér, J. Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation. J. Vis. Exp. (143), e58824, doi:10.3791/58824 (2019).

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