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Identification des souris et des répertoires d’anticorps humain par séquençage de prochaine génération
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Identification des souris et des répertoires d’anticorps humain par séquençage de prochaine génération
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08:51 min
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March 15, 2019
DOI:
1Graduate School of Life and Environmental Sciences,University of Tsukuba, 2Center for Influenza Virus Research,National Institute of Infectious Diseases, 3School of Science and Technology,Kwansei Gakuin University, 4Department of Pathology,National Institute of Infectious Diseases, 5Division of Infectious Diseases Pathology, Department of Global Infectious Diseases,Tohoku University Graduate School of Medicine, 6Division of Molecular Pathobiology, Research Center for Zoonosis Control,Hokkaido University, 7Division of Collaboration and Education, Research Center for Zoonosis Control,Hokkaido University, 8Global Station for Zoonosis Control, GI-CoRE,Hokkaido University, 9Department of Immunology,National Institute of Infectious Diseases, 10Faculty of Life and Environmental Sciences,University of Tsukuba
Summary
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Nous décrivons ici les protocoles pour l’analyse et la visualisation de la structure et la constitution de répertoires entiers d’anticorps. Il s’agit de l’acquisition de vastes séquences d’ARN d’anticorps moyen du séquençage de prochaine génération.
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Sun, L., Kono, N., Toh, H., Xue, H., Sano, K., Suzuki, T., Ainai, A., Orba, Y., Yamagishi, J., Hasegawa, H., Takahashi, Y., Itamura, S., Ohnishi, K. Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing. J. Vis. Exp. (145), e58804, doi:10.3791/58804 (2019).
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