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卡瑞津、S100 蛋白和蛋白质基因产物9.5 的免疫修饰直肠吸吮活检诊断
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Diagnosis of Hirschsprung’s Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
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卡瑞津、S100 蛋白和蛋白质基因产物9.5 的免疫修饰直肠吸吮活检诊断

DOI:
10.3791/58799-v

•

05:45 min

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April 26, 2019

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, , , ,
1Department of Pediatric Surgery, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 2Division of Gastroenterology, Hepatology and Nutrition,Cincinnati Children’s Hospital Medical Center

Chapters

  • 00:04Title
  • 00:40Rectal Suction Biopsy (RSB) Section Preparation
  • 01:52Antigen Retrieval and Tissue Staining
  • 04:25Results: Representative RSB Immunohistochemical Analysis
  • 05:09Conclusion

Summary

자동 번역

자동 번역

该方案描述了对卡莱丁、S100 蛋白和蛋白质基因产物9.5 进行免疫染色直肠抽吸活检的过程。这种新型辅助诊断方法对先天性巨结肠病具有较好的敏感性和特异性。

Tags

Hirschsprung’s DiseaseImmunostainingRectal Suction BiopsyCalretininS100 ProteinProtein Gene Product 9.5DiagnosisSensitivitySpecificityParaformaldehydeDehydrationParaffin EmbeddingMicrotomeAntigen RetrievalHydrogen PeroxideBovine Serum AlbuminPrimary AntibodyPolymer EnhancerSecondary Antibody

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