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Isolering af voksen rygmarven kerner for massivt Parallel Single-kernen RNA sekventering
1Spinal Circuits and Plasticity Unit,National Institute of Neurological Disorders and Stroke, 2Bioinformatics Section, Information Technology Program,National Institute of Neurological Disorders and Stroke, 3Laboratory of Cochlear Development,National Institute on Deafness and Other Communication Disorders, 4Single Cell Analysis Facility,Frederick National Laboratory
Chapters
- 00:04Title
- 00:43Preparation of the Spinal Cord
- 02:03Detergent-mechanical Cell Lysis
- 03:25Homogenization and Sucrose Density Gradient
- 05:11Results: Representative Results of Nuclei Isolated from the Adult Mouse Spinal Cord
- 05:51Conclusion
Vi præsenterer her, en protokol for at hurtigt isolere høj kvalitet kerner fra frisk eller frossen væv til downstream massivt parallel RNA sekvensering. Vi medtager vaskemiddel-mekaniske og hypotonic-mekaniske væv forstyrrelser og celle lysis muligheder, som begge kan bruges til isolering af kerner.
