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Immunology and Infection

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JoVE Journal Immunology and Infection
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
 

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Article DOI: 10.3791/58407-v • 08:36 min • November 1st, 2018
November 1st, 2018 •
1, 1, 1, 1, 1, 1
1Department of Microbiology and Infection Control, Osaka Medical College

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Real-time quantitative polymerase chain reaction analysis combined with reverse transcription (RT-qPCR) has been widely used to measure the level of RNA virus infections. Here we present a direct RT-qPCR assay, which does not require an RNA purification step, developed for the quantification of several RNA viruses, including dengue virus.

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Dengue Virus RNA Real-time Quantitative Polymerase Chain Reaction Infectious Disease Dengue Fever Chikungunya Fever Viral RNA Quantification High Throughput Drug Screening Clinical Diagnosis Biosafety Cabinet Laboratory Infection DNA Template In Vivo Transcription Reaction DNAase RNA Purification Kit Capture Buffer Betamarcaptoethanol Ethanol RNA Purification Column
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