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基于板块的秀丽线虫规模化栽培: 糖尿病代谢变化研究的样品制备
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Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes
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基于板块的秀丽线虫规模化栽培: 糖尿病代谢变化研究的样品制备

6,174 Views

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05:08 min

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August 24, 2018

DOI:

10.3791/58117-v

DOI:
10.3791/58117-v

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05:08 min
August 24, 2018

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8 Views
, , , , ,
15th Medical Department, Medical Faculty Mannheim,Heidelberg University, 2Department of Internal Medicine,Heidelberg University, 3German Center for Diabetes Research (DZD), 4European Center for Angioscience, Medical Faculty Mannheim,Heidelberg University

Summary

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本协议描述了一种在固体培养基上大规模培养秀丽线虫的方法。作为液体培养的替代品, 该协议允许在板基培养条件下获得不同尺度的参数。这增加了结果的可比性通过省略液体和固体媒介文化之间的形态学和新陈代谢的区别。

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Kohl, K., Fleming, T., Acunman, K., Hammes, H., Morcos, M., Schlotterer, A. Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes. J. Vis. Exp. (138), e58117, doi:10.3791/58117 (2018).

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