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用 RNA原位杂交法对形态学组织环境中的遗传变异、短靶点和突变进行可视化
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August 14, 2018
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在这里, 我们描述了原位杂交检测, 使敏感和特定的检测序列短于50核苷酸的单核苷酸分辨率在单细胞水平。该检测可以手动或自动执行, 可以在组织上下文中实现拼接变体、短序列和突变的可视化。
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Genetic VariantsShort TargetsPoint MutationsRNA In Situ HybridizationCell Type ExpressionSplice Variants신경과학OncologyExon JunctionsSensitivitySpecificitySingle CellsFormalin FixationParaffin EmbeddingMicrotome SectioningTarget RetrievalWash BufferHematoxylin CounterstainProbe HybridizationAmplification Reagents
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Anderson, C. M., Laeremans, A., Wang, X. M., Wu, X., Zhang, B., Doolittle, E., Kim, J., Li, N., Pimentel, H. X. Y., Park, E., Ma, X. Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay. J. Vis. Exp. (138), e58097, doi:10.3791/58097 (2018).
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