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哺乳類の Mrna の翻訳開始複合体形成の Toeprinting 分析

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Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

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哺乳類の Mrna の翻訳開始複合体形成の Toeprinting 分析

12,264 Views

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10:37 min

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May 10, 2018

DOI:

10.3791/57519-v

DOI:

10.3791/57519-v

•

10:37 min

May 10, 2018

•

14 Views

Joseph A. Ross¹, Nehal Thakor^1,2,3

¹Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute,University of Lethbridge, ²Department of Neuroscience and the Canadian Centre for Behavioral Neuroscience (CCBN),University of Lethbridge, ³Arnie Charbonneau Cancer Institute,University of Calgary

Summary

体外の能力を計測する Toeprinting の目的は、リボソームさまざまな条件の下でフォームの翻訳開始複合体に RNA を転写しました。このプロトコルは toeprinting 哺乳類の RNA のための方法を記述してキャップ依存、IRES 駆動の両方の翻訳を勉強する使用ことができます。

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