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基因靶向随机诱变在裂变酵母中选择染色质稳定蛋白酶突变体

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Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

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基因靶向随机诱变在裂变酵母中选择染色质稳定蛋白酶突变体

10,490 Views

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07:18 min

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May 15, 2018

DOI:

10.3791/57499-v

DOI:

10.3791/57499-v

•

07:18 min

May 15, 2018

•

2 Views

Hogyu David Seo¹, Daeyoup Lee¹

¹Department of Biological Sciences,Korea Advanced Institute of Science and Technology

Summary

本文介绍了一种用于裂变酵母靶基因随机诱变的详细方法。例如, 我们针对的是rpt4+, 它对19S 蛋白酶体的亚基进行编码, 并筛选出破坏染色质的突变。

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