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分子互补亲和纯化 (BiCAP) 对多蛋白信号络合物的解剖
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06:45 min
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June 15, 2018
DOI:
1The Kinghorn Cancer Centre,Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences,University of Copenhagen, 3RCSI Molecular Medicine,Royal College of Surgeons in Ireland, 4Department of Epigenetics,Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences,University of New South Wales, 6St Vincent’s Hospital Clinical School,University of New South Wales, 7School of Medicine and Medical Science,University College Dublin
Summary
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本手稿描述了分子互补亲和纯化 (BiCAP) 的协议。这种新方法促进了任何两种相互作用的蛋白质的特定隔离和下游蛋白质组特征, 同时排除了不复杂的个体蛋白质以及与竞争的结合伙伴形成的复合体。
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Hastings, J. F., Han, J. Z., Shearer, R. F., Kennedy, S. P., Iconomou, M., Saunders, D. N., Croucher, D. R. Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP). J. Vis. Exp. (136), e57109, doi:10.3791/57109 (2018).
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