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Developmental Biology

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JoVE Journal Developmental Biology
Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification
 

Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification

Article DOI: 10.3791/56888-v • 10:15 min • December 9th, 2017
December 9th, 2017 •
1, 1,2
1Department of Pediatrics/Child Neurology, Amsterdam Neuroscience, VU University Medical Center, 2Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

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요약
Automatic Translation

We describe a simplified 3D differentiation protocol for hPSCs, using defined medium and reduced growth factors, capable of generating cell aggregates with early neuroepithelial structures and positive for cerebellar-associated markers, as well as an optional 2D modification for differentiating cells as a monolayer to generate functional neurons.

Tags

3D Cerebellar Differentiation Protocol 2D Modification Stem Cells Brain Disorders Neural Protocols Technician HPSCs Differentiation Protocols Extrinsic Patterning Factors Pluripotent Stem Cells
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