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人诱导多能干细胞的前脑型脑 Organoids 标准化和可再生性的生成
JoVE 신문
발생학
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JoVE 신문 발생학
Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
DOI:

10:25 min

January 23, 2018

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Chapters

  • 00:05Title
  • 00:55iPSC Aggregate Generation
  • 02:30Anterior Neuroectoderm Induction
  • 03:51Neuroectodermal Aggregates embedding within a Matrix Scaffold
  • 05:49Forebrain-type Organoid Generation, Fixation, and Validation
  • 07:58Results: Representative Forebrain-type Organoid Validation
  • 09:36Conclusion

Summary

자동 번역

脑 organoids 代表了一个新的模型系统来研究早期人脑发育体外。本文提供了详细的方法, 以有效地产生同种背前脑型 organoids 的人诱导多能干细胞, 包括关键的表征和验证步骤。

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