Synaptic currents can be recorded focally from visualized synaptic boutons at the Drosophila third instar larvae neuromuscular junction. This technique enables monitoring the activity of a single synaptic bouton.
Vasin, A., Bykhovskaia, M. Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction. J. Vis. Exp. (127), e56493, doi:10.3791/56493 (2017).