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Imaging di attività neurale nella corteccia somatosensoriale primaria utilizzando Thy1-topi transgenici GCaMP6s
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January 07, 2019
DOI:
1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Department of Anatomy and Cell Biology,Indiana University School of Medicine, 2Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA,General Hospital of Jinan Military Region, 3Department of Orthopedics, Shandong Cancer Hospital,Shandong University, 4Department of Neurobiology, Institute of Basic Medical Sciences,Academy Military Medical Sciences
Summary
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Descriviamo una procedura sperimentale per la misura di attività neuronale attraverso finestre ottiche dual sopra bilaterale corticies somatosensoriale primaria (S1) in Thy1-GCaMP6s topi transgenici utilizzando 2 fotoni (2P) microscopia in vivo.
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Lin, X., Zhao, T., Xiong, W., Wen, S., Jin, X., Xu, X. Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice. J. Vis. Exp. (143), e56297, doi:10.3791/56297 (2019).
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