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Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
1Department of Metabolic Biology,John Innes Centre, Norwich Research Park, 2Department of Botany,University of Wisconsin, Madison, 3Department of Biochemistry and Molecular Biology,Saitama University, 4Precursory Research for Embryonic Science and Technology (PRESTO),Japan Science and Technology Agency (JST), 5Department of Cell and Developmental Biology,John Innes Centre, Norwich Research Park
Chapters
- 00:05Title
- 00:44Arabidopsis Leaf Detachment
- 01:38Fluorescence Microscopy
- 03:06Data Collection
- 05:39Time-course Video Creation
- 06:39Results: Analysis of GCaMP3 Fluorescence to Detect Aphid-elicited Calcium Signals at the Feeding Site in Detached Arabidopsis Leaves
- 07:30Conclusion
This protocol outlines a simple method for analyzing calcium signals in plants generated by feeding hemipteran insects, such as aphids. Arabidopsis thaliana transformed with the GFP calcium biosensor GCaMP3 allow for the real-time in vivo imaging of calcium dynamics with a high temporal and spatial resolution.
