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在细胞分化过程中光介导的可逆调节丝裂原活化蛋白激酶途径<em>爪蟾</em>胚胎发育
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09:32 min
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June 15, 2017
DOI:
1Department of Biochemistry,University of Illinois at Urbana-Champaign, 2Department of Comparative Biosciences,University of Illinois at Urbana-Champaign, 3Neuroscience Program,University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign
Summary
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该协议描述了在细胞分化和非洲爪蟾胚胎发育过程中调节丝裂原活化蛋白激酶（MAPK）活性的光遗传策略。这种方法允许哺乳动物细胞培养物和多细胞活生物体如非洲爪蟾胚胎中具有高空间和时间分辨率的可逆激活MAPK信号通路。
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Cite this Article
Krishnamurthy, V. V., Turgeon, A. J., Khamo, J. S., Mondal, P., Sharum, S. R., Mei, W., Yang, J., Zhang, K. Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development. J. Vis. Exp. (124), e55823, doi:10.3791/55823 (2017).
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