JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Tweerichtings retrovirale integratie site PCR methodologie en kwantitatieve data analyse werkstroom
10,587 Views
•
12:53 min
•
June 14, 2017
DOI:
1UCLA AIDS Institute,University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics,University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics,University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups,Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine,University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine,The Ohio State University (OSU)
Summary
Automatically generated
Dit manuscript beschrijft de experimentele procedure en software analyse voor een bidirectionele integratie site analyse die tegelijkertijd upstream en downstream vector-host junction DNA kan analyseren. Bidirectionele PCR producten kunnen worden gebruikt voor elk downstream sequencing platform. De resulterende gegevens zijn bruikbaar voor een high-throughput, kwantitatieve vergelijking van geïntegreerde DNA-doelen.
Read Article
Cite this Article
Suryawanshi, G. W., Xu, S., Xie, Y., Chou, T., Kim, N., Chen, I. S. Y., Kim, S. Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow. J. Vis. Exp. (124), e55812, doi:10.3791/55812 (2017).
Copy